Reduced volume for direct PCR amplification of blood reference samples using Identifiler® Direct and Globalfiler™ Express assays
نویسندگان
چکیده
منابع مشابه
An evaluation of direct PCR amplification
AIM To generate complete DNA profiles from blood and saliva samples deposited on FTA® and non-FTA® paper substrates following a direct amplification protocol. METHODS Saliva samples from living donors and blood samples from deceased individuals were deposited on ten different FTA® and non-FTA® substrates. These ten paper substrates containing body fluids were kept at room temperature for vary...
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چکیده ندارد.
Direct PCR from Whole Blood
Researchers often purify DNA from blood samples prior to performing PCR because it is believed that blood constituents and the reagents commonly used to preserve blood samples (e.g., anticoagulants) interfere with PCR. In order to save the time and expense required for template purification, several methods have been reported for direct PCR of blood samples. These include microwave irradiation,...
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ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2017
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2017.09.118